Klearia is a spin-off born at the Laboratory for Photonics and Nanostructures (LPN-CNRS) from the discovery of an innovative technology for bonding glass microfluidic chips.
Klearia overcomes a major technological limitation by bonding at a low temperature between 100°C and 300°C. This way Klearia is able to integrate a wider range of materials and devices. For example, our exclusive and patented technology lets us develop carbon instrumentations on a chip and potentially nanostructured metallic structures.
Biology, health, environment and agribusiness are industries with great interest in the development of microfluidic sensors allowing electrochemical detection. This innovation permits a complete process to be performed inside the chip. You can find an adapted solution to your microfluidic needs through our custom made services. Klearia assiduously works to create valuable new products and processes. With our distinct expertise, Klearia aims to tackle challenges related to sustainable development of responsible chemistry and conservation of natural resources.
Our projects and collaborations
FLAshMoB : FunctionaL Amyloid chimera for Marine Biosensing
Klearia is working on the FlashMob project funded by ANR in the framework of the H2020 Martera program.
The aim is to develop small, portable, easy to use, and robust biosensor platforms to monitor marine contaminants with high sensitivity and specificity, offering the ability to work on site and in a wide range of matrices. The bases for the development of the miniaturized sensors are chimera proteins endowed with both the adhesive properties of a self-assembling amyloid moiety and the recognition ability of specific proteins, such as:
laccase, an oxidative enzyme useful for sensing aromatic pollutants, polycyclic aromatic hydrocarbons and endocrine disrupting chemicals;
Arsenate reductase, an extremophilic enzyme to be used for specific arsenic detection;
Antibodies against marine toxins from algae, such as saxitoxin and domoic acid;
Glutathione S-transferase (GST), a multifunctional protein useful for the detection of the total heavy metals content.
MIP_WQT : MIPs as sorbents or recognition elements for pollutants monitoring in waters
The project aims to implement innovative molecularly imprinted polymers (MIPs) based strategies, with integrated and complementary analytical and bioanalytical approaches enabling more effective monitoring of relevant pollutants at ultra-trace level in waters in order to:
enable a more reliable- and representative-monitoring of relevant pollutants at ultra-trace level;
improve the understanding of mode of action of the selected pollutants focusing on endocrine disruption;
enhance the knowledge of cocktail effects in natural environments;
sustain the innovation and competiveness in environmental metrology at the national level.
MIPs molecules are synthesized by Klearia’s partners. They are then used as electrodes modifiers for quantitative detection of pollutants or as a sorbent selective extraction of real samples thus rendering their quantitative analysis more reliable and sensitive using electrochemical sensors and Lab on a Chip.
SURIMI: SUrface plasmon Resonance for In situ detection of Metallic Ions
The aim of the SURIMI project is to develop a multi-detection metallic elements sensor capable of performing robust and sensitive analyses in settings ranging from a laboratory environment to the harshest conditions (e.g. deep ocean and/or polar seas). Two applications are targeted:
1. Pollution monitoring of Cd, Hg, Pb and Ni: one laboratory and one on-field system will be developed and industrially valorized by Klearia.
2. Oceanographic studies on Ni, Cu, Zn, and Hg: one ocean underwater system allowing for a rapid screening of these elements in the seawater will be developed and tested.
To achieve this objective, the SURIMI project aims to combine cutting-edge technologies such as plasmonic, imprinted polymers and microfluidics. The systems will consist in an SPRI (Surface Plasmon Resonance Imaging) transducer, functionalized by new Ion Imprinted Polymers (IIPs) specific to the target elements. They will be capable of assaying four different metallic elements at concentration as low as nM every 5 min.
Klearia is one of the major partners of BACTERI’EAU project funded by ANR. BACTERI’EAU is public-private collaborative project associating two academic teams and two SMEs with expertise and know-how in organic synthesis, electrochemical sensing, surface modifications, microfluidics, microbiology, and an end-users committee. BACTERI’EAU aims at developing a new microfluidic device using an innovative electrochemical sensor for in situ and real time monitoring of bacteriological quality of water. Two sensors will be developed and integrated in the same chip. One will measure total E. coli and the other will target the most clinically relevant pathogenic ones (Shiga toxin-producing E. coli) in a water sample.